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Proposal for dating analysis of the post 50 000 year old occupation layers at Klipdrift Shelter

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Overview We are requesting permission to export 7 organic (charcoal and bone) samples collected from the Middle Stone Age site of Klipdrift Shelter (KDS) for radiocarbon (via accelerator mass spectrometry - AMS) dating by Beta Analytic in Miami, Florida, United States of America https://www.radiocarbon.com/ . The aim of this research is to provide ages for the upper occupation layers that have not been dated using other radiometric dating methods. These upper units include the terminal layers of occupation of Klipdrift Shelter. The youngest layer (layer PAN/PAO) that has been dated (using single-grain optically stimulated luminescence - OSL) dates to ~50 ka (thousand years ago) (Henshilwood et al. 2014). The base of the Klipdrift Shelter sequence dates to ~ 71 ka. The layers containing artefacts belonging to the Howiesons Poort technotradition (HP) at KDS date to between 65.5 ± 4.8 ka to 59.4 ± 4.6 ka. Based on observations during excavation and material analysis of the less than 50 ka old material, it is not clear what the ages of these deposits are. They do not contain cultural artefacts indicative of a specific technotradition, although sample sizes are currently quite small. A pilot study on ancient DNA (aDNA) preservation at KDS (Meyer, HWC REPORT Number: CASE #:19100205AS1114E) found that no aDNA was preserved in the older than 50 ka layers, but aDNA was recovered from one of the younger layers (layer PAM). This differential preservation might be related to age, as younger materials are more likely to have better preservation (Meyer, personal communication). Thus the layers above the HP at KDS could potentially be of any age, even relatively recent, for which radiocarbon dating is particularly suited as it falls into the age range of the method. Analyses of micromammals (NEL, HWC permit number: CASE #: 20092306SB1102E) from the post 50 ka layers show distinct differences in species (and therefore rainfall seasonality) between certain layers, suggesting that considerable time had passed between deposition of certain of the upper layers. It is imperative to establish the time period represented in order to better interpret current materials and inform future excavation strategies. Samples were collected during the 2018 excavation season. Methodology “The Accelerator Mass Spectrometry (AMS) technique for radiocarbon dating accounts for a substantial number of the analysis requests Beta Analytic receives each day. There can be considerable advantages to using the AMS technique in many dating applications, making it possible to extend radiocarbon dating into many new areas of research. AMS also permits applications in important situations that cannot be dated by the radiometric dating technique. Beta Analytic has been providing routine AMS radiocarbon dating services to the international scientific community since 1983. The company routinely delivers AMS radiocarbon dating results within 14 business days. AMS Lab Procedure The AMS measurement is done on graphite produced by hydrogen reduction of the CO2 sample over a cobalt catalyst. The CO2 is obtained from the combustion of the sample at 800°C+ under a 100% oxygen atmosphere. The CO2 is first dried with methanol/dry ice then collected in liquid nitrogen for the subsequent graphitization reaction. The identical reaction is performed on reference standards, internal QA samples, and backgrounds to ensure systematic chemistry. The analytical result (“BP” or “pMC”) is obtained by measuring sample C14/C13 relative to the C14/C13 in Oxalic Acid II (NIST-4990C) in one of Beta Analytic’s multiple in-house particle accelerators using SNICS ion source. Quality assurance samples are measured along with the unknowns and reported separately in a “QA report“. The radiocarbon dating lab requires results for the QA samples to fall within expectations of the known values prior to accepting and reporting the results for any given sample. The AMS result is corrected for total fractionation using machine graphite d13C. The d13C reported for the sample is obtained by different ways depending upon the sample material. Solid organics are sub-sampled and converted to CO2 with an elemental analyzer (EA). Water and carbonates are acidified in a gas bench to produce CO2. Both the EA and the gas bench are connected directly to an isotope-ratio mass spectrometer (IRMS). The IRMS performs the separation and measurement of the CO2 masses (44, 45, and 46) and calculation of the sample d13C.”

ApplicationDate: 

Tuesday, March 1, 2022 - 09:31

CaseID: 

18032

OtherReferences: 

ReferenceList: 

CitationReferenceType
Armitage, Simon, Christopher Henshilwood, and Karen Loise Van Niekerk. 2014. "Single-grain OSL dating of the Howiesons Poort layers at Klipdrift Shelter, southern Cape, South Africa." In 14th Congress of the Pan African Archaeological Association for Prehistory and Related Studies and the 22nd Biennial Meeting of the Society of Africanist Archaeologists. Johannesburg.
Delagnes, Anne, Patrick Schmidt, Katja Douze, Sarah Wurz, Ludovic Bellot-Gurlet, Nicholas J. Conard, Klaus G. Nickel, Karen L. van Niekerk, and Christopher S. Henshilwood. 2016. 'Early Evidence for the Extensive Heat Treatment of Silcrete in the Howiesons Poort at Klipdrift Shelter (Layer PBD, 65 ka), South Africa', PLOS ONE, 11: e0163874
Douze, Katja, Anne Delagnes, Sarah Jacoba Deborah Wurz, and Christopher Henshilwood. 2018. 'The Howiesons Poort lithic sequence of Klipdrift Shelter, southern Cape, South Africa', PLOS ONE, 13:e0206238: 1-24.
Harvati, Katerina, Catherine C. Bauer, Frederick E. Grine, Stefano Benazzi, Rebecca Rogers Ackerman, Karen Loise Van Niekerk, and Christopher Henshilwood. 2015. 'A human deciduous molar from the Middle Stone Age (Howiesons Poort) of Klipdrift Shelter, South Africa', Journal of Human Evolution, 82: 190-96.
Henshilwood, Christopher S., Karen L. van Niekerk, Sarah Wurz, Anne Delagnes, Simon J. Armitage, Riaan F. Rifkin, Katja Douze, Petro Keene, Magnus M. Haaland, Jerome Reynard, Emmanuel Discamps, and Samantha S. Mienies. 2014. 'Klipdrift Shelter, southern Cape, South Africa: preliminary report on the Howiesons Poort layers', Journal of Archaeological Science, 45: 284-303.
Reynard, Jerome, Emmanuel Discamps, Shaw Badenhorst, Karen Loise Van Niekerk, and Christopher Henshilwood. 2015. 'Subsistence strategies in the southern Cape during the Howiesons Poort: Taphonomic and zooarchaeological analyses of Klipdrift Shelter, South Africa', Quaternary International.
Reynard, Jerome, and Christopher Henshilwood. 2017. 'Subsistence strategies during the Late Pleistocene in the southern Cape of South Africa: Comparing the Still Bay of Blombos Cave with the Howiesons Poort of Klipdrift Shelter', Journal of Human Evolution, 108: 110-30
Reynard, Jerome P., Emmanuel Discamps, Sarah Wurz, Karen L. van Niekerk, Shaw Badenhorst, and Christopher S. Henshilwood. 2016. 'Occupational intensity and environmental changes during the Howiesons Poort at Klipdrift Shelter, southern Cape, South Africa', Palaeogeography, Palaeoclimatology, Palaeoecology, 449: 349-64.
Roberts, Patrick, Christopher S. Henshilwood, Karen L. van Niekerk, Petro Keene, Andrew Gledhill, Jerome Reynard, Shaw Badenhorst, and Julia Lee-Thorp. 2016. 'Climate, Environment and Early Human Innovation: Stable Isotope and Faunal Proxy Evidence from Archaeological Sites (98-59ka) in the Southern Cape, South Africa', PLOS ONE, 11: e0157408.
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