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Archaeological cattle remains from KZN Iron Age sites

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Case Type: 

ProposalDescription: 

Between 0.5g and 2g of bone or tooth will be ground to powder. DNA will be extracted using a guanidinium thiocyanate and silica method that has been shown to be the most efficient of the available methods for DNA recovery. Next-generation DNA sequencing libraries for the illumina MiSeq platform will be prepared and complete mitochondrial genomes (~16,500bp) will be captured with an in-solution hybridisation method. Normalised captured libraries and their associated negative controls will be sequenced on the MiSeq platform. Genomes will be assembled using the Geneious software packages. Upon publication, DNA sequences will be submitted to the online database GenBank and copies of publications lodged with AMAFA and the KwaZulu Natal Museum

Expanded_Motivation: 

This work proposed here will address several questions about the relationship among southern Africa's cattle. Specifically, we will investigate the relationships between the cattle of the Later Stone Age, the Early Iron Age and the Late Iron Age. Did Khoe and Bantu acquire their cattle from the same original source, or are they semi-independent populations? The development of in-solution DNA hybridisation capture methods and next -generation DNA sequencing techniques has allowed us, for the first time, to analyse the poorly preserved DNA in archaeological remains from southern Africa. We have successfully recovered DNA from Later Stone Age cattle from the Western Cape Province, Iron Age cattle from the Limpopo province and Lesotho. We are therefore hopeful that we will be able to recover DNA from cattle from KwaZulu natal.

ApplicationDate: 

Monday, March 17, 2014 - 15:46

CaseID: 

5115

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